Imethod doa 2.0 1710210-0

A Rapid iMethod™ Test for Drugs of Abuse Screening iMethod™ Test for Drugs of Abuse Screening Version 2.0 for Cliquid® Software
Liquid Chromatography coupled to Tandem Mass Spectrometry
LC/MS/MS system. This iMethod™ Test includes both an (LC/MS/MS) has quickly become the technique of choice for both iMethod™ test for the screening of 700 drugs of abuse in a screening and confirmation due to elimination of derivatization single run as well as an MRM catalogue containing over 1250 and simplification of sample preparation, while offering better drugs of abuse and related compounds, with up to 6 transitions sensitivity and selectivity, shorter run times, and the ability to per compound. The MRM catalogue can be used to create analyze wider polarities and molecular weight range of additional custom screening and/or quantification methods. Please note that the AB SCIEX 1250 compound forensic drug library required for confirmation is sold separately. The following description outlines the instrument requirements and expected results obtainable from the AB SCIEX iMethod™ A number of example sample preparation procedures are Test for the Screening of Drugs of Abuse in plasma, blood or provided, based upon the matrix, the degree of clean up required urine when using an AB SCIEX 3200 QTRAP® instrument. The and/or the preferred mode of ionization. These range from a use of this instrument allows the utilization of an information simple extraction and dilution for urine to liquid-liquid for blood dependent acquisition consisting of an MRM survey scan that and plasma to solid phase extraction for plasma and urine. triggers product ion dependent scans that can be used for Internal standards of Diazepam-D5 and Doxepin-D3 at known compound confirmation via library searching. This method has concentration are added during sample preparation to monitor also been developed and verified for use with 4000 QTRAP® Figure 1: Chromatogram of 300 drugs of abuse and pharmaceuticals detected by LC/MS/MS in Multiple Reaction Monitoring with highest
selectivity and sensitivity
Table 1: List of compounds in the 700 compound drug screening method.
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
Compound name
An example chromatogram for drugs of abuse and screening is shown in Figure 1 to highlight the possibility of detecting hundreds of compounds in a single experiment. An example reports generate automatically after analysis by Cliquid® Figure 2: Example screening report.
System Requirements
While the information provided above outlines the instrument requirements and expected results obtainable from the In order to run this method as outlined above, the following AB SCIEX iMethod™ Test for the Analysis of Drugs of Abuse, please note that the results obtained do require some experience • An AB SCIEX 3200 QTRAP® or 4000 QTRAP® LC/MS/MS with LC/MS/MS and sample preparation procedures. As such, web-based and on-site training are available to assist in the deployment of the iMethod™ Test and are recommended for A Shimadzu Prominence 20A LC System with reservoir tray inexperienced users. Please consult your local sales and bottles, system controller CBM-20A, 100 µL mixer, 2 isocratic pumps LC-20AD, 3 channel degasser Autosampler SIL-20AC, column oven CTO-20AC or Agilent 1100/1200 LC Important Note
system with binary pump G1312A (without static mixer), well plate auto sampler, and thermostated column oven. The iMethod™ Test described above has been designed by • LC/MS grade water, acetonitrile, ammonium formate and AB SCIEX to provide the sample prep and instrument parameters required to accelerate the adoption of this method for routine testing. This method is provided for information purposes only. The performance of this method is not guaranteed due to • A Restek pre-column, 5µm 60Å, PFP Propyl, 10 x 2.1 mm many different potential variations, including instrument • A Restek analytical column, 5µm 60Å, PFP Propyl Column, 50 performance, tuning, and maintenance, chemical variability and procedures used, technical experience, sample matrices, and • SPE cartridges, 200mg Chromabond® Macherey-Nagel environmental conditions. It us up to the end user to make adjustments to this method to account for slight differences in A centrifuge able to accommodate Eppendorf tubes and run at equipment and/or materials from lab to lab as well as to determine and validate the performance of this method for a • Pipettes and standard laboratory glassware given instrument and sample type. Please note that a working knowledge of Analyst® Software may be required to do so. The purchase and use of certain of the chemicals listed below Ordering Information
may require the end user to possess any necessary licenses, permits or approvals, if such are required in accordance with Product Name
Part Number
local laws and regulations. It is the responsibility of the end user iMethod™ Test for Drugs of Abuse Screening to purchase these chemicals from a licensed supplier, if required in accordance with local laws and regulations. The suppliers and part numbers listed below are for illustrative purposes only and may or may not meet the aforementioned local requirements. AB SCIEX is not responsible for user’s compliance with any statute or regulation, or for any permit or approval required for user to implement any iMethod™ procedure. The information included in this product is intended for reference and research purposes only. AB SCIEX offers no guarantee as to the quality or suitability of this data and suitability of the information included in this (Library, database, etc.) for use with For Research Use Only. Not for use in diagnostic procedures.
2010 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.

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