Sartorius.be

Technical data and operating instructions
Vivaspin® Turbo 15 – Introduction
Storage conditions|shelf life
Equipment Required
For use with centrifuge
1. Centrifuge with swing bucket or fixed Introduction
Vivaspin® Turbo 15 centrifugal concentrators offer the optimal solution to any concentra- Carrier Required
tion or buffer exchange application with Highest flow rates are achieved due to their double verticle membranes which minimize protein polarization and subsequent fouling of the membrane. Additionally, their sleek internal profile ensures maximum process speeds right down to the last 150 µl.The UV joining technology allows for a smooth joint housing, allowing complete sample recovery from the unique pipette friendly deadstop pocket. The dead stop pocket (patent pend- ing) is an impermeable concentrate pocket surface, thereby eliminating the risk of filtra- The Vivaspin® Turbo 15 devices are the opti- mal solution for protein concentration from dilute samples e.g. after chromatography, cell culture supernatants or fermentation Operation
Vivaspin® Turbo 15 concentrators can be used in swing bucket or fixed angle rotors accepting standard conical bottom tubes. In a single spin, solutions can be concentrated up to 150 +. Samples are typically concen- Equipment Required
Equipment required
Vivaspin® Turbo 15
Centrifuge
Rotor type
Rotor compatibility
Please note: Vivaspin® Turbo 15 (30 mm + 118 mm) is designed to fit into rotors that with TS-5.1-500 swing-out rotor with BUC 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.
These devices are not designed to fit into please use the Vivaspin® 15, which can be Operation
Desalting | Buffer Exchange
1. Concentrate sample to desired level.
recovery select a MWCO at least 50% smaller than the molecular size of the species of 3. Refill concentrator with an appropriate 4. Concentrate the sample again and repeat contaminating microsolute is sufficiently reduced. Typically, 3 wash cycles will remove used, angle concentrator so that the printed in table 2, taking care not to exceed the achieved, (see table 3a and 3b for guide to concentration times), remove assembly Technical Specifications
Table 1: Technical Specifications
Vivaspin® Turbo 15
Dimensions
Total Length
Concentrator Capacity
Swing bucket rotor
Materials of construction
Concentrator insert
Table 2: Recommended Spin Speed (xg)

Vivaspin® Turbo 15
Centrifuge
* Dead stop volume as designed in moulding tool. This volume may vary depending on sample, sample concentration, operation temperature and centrifuge rotor.
Usage Tips
1. Flow Rate
3. Sterilisation of Polyethersulfone
Filtration rate is affected by several para- Membranes
meters, including MWCO, porosity, sample concentration, viscosity, centrifugal force and temperature. Expect significantly longer spin times for starting solutions with over To sterilise, use a 70% ethanol solution or 5% solids. When operating at 4°C. flow rates 4. Chemical Compatibility
glycerine will take up to 5 times longer to Vivaspin® concentrators are designed for use concentrate than samples in a predominantly with biological fluids and aqueous solutions. For chemical compatibility details, refer to 2. Pre-rinsing
Membranes fitted to Vivaspin® concentrators Sodium azide. Should these interfere with analysis they can be removed by rinsing fill volume of buffer solution or deionised water through the concentrator. Decant filtrate and concentrate before processing sample pre-rinsed device immediately, store it in the refrigerator with buffer or water covering the membrane surface. Please do not allow Performance Characteristics
Table 3: Performance Characteristics Vivaspin® Turbo™ 15
Time to concentrate up to 20x [min.] at 20°C and solute recovery %
Rotor
Chemical Compatibility
Table 4: Chemical Compatibility (2hr contact time)
Solutions
Solutions
Compatible pH range
pH 1–14
Compatible pH range
pH 1–14
OK = Acceptable ? = Questionable NO = Not recommended Ordering Information
Qty per box
Prod. no.
Other Products
Sample volume
Membranes available
37079 Goettingen, GermanyPhone +49.551.308.0 been bleached without any use of chlorine

Source: http://www.sartorius.be/fileadmin/fm-dam/sartorius_media/Bioprocess-Solutions/Lab_Technologies/Lab_Purification/Ultrafiltration/Manuals/Manual_VivaspinTurbo15_SLU6126-e.pdf