Renxiao wang, ph

Shanghai Institute of Organic Chemistry Wen Liu, Ph.D.

Research
 Natural Product Biosynthesis (Genetics, Biochemistry and Chemistry) Interests
 Combinatorial Biosynthesis for Production Improvement and Structural  Genome and Transcriptome Mining for Discovery of Novel Microbial Natural Professional
2003–present: Professor, Shanghai Institute of Organic Chemistry, Chinese
Academy of Sciences, China
Experiences
2001-2003: Research Associate, School of Pharmacy, University of Wisconsin-
Madison, United States
2000–2001: Postdoctoral Fellow, Department of Chemistry, University of California,
Davis, United States
Education
1994-2000: M.S. & Ph.D., Chinese Academy of Medical Sciences (Peking Union
Medical College), China

 Department: Institute of Medicinal Biotechnology
 Major: Molecular Biology & Medical Biochemistry
 Director:
Prof. Yuan Li, Chinese Academy of Medical Sciences Prof. Ben Shen, University of California, Davis, United States  Thesis title: “Biosynthesis of the Enediyne Antitumor Antibiotic C-1027” 1988-1992: B.S., Sichuan University, China
 Department: Biotechnology
 Major: Microbiology
Honors and
2005: Distinguished Young Scholar Funded by CNSF

2005: Pujiang Scholar Funded by STCSM
2005: Rising-Star Scholar Funded by STCSM
Professional
Associations
Member of the 5th Editorial Board of “Chinese Journal of Organic Chemistry” Member of the 8th Editorial Board of “Chinese Journal of Antibiotics”
1. Smanski, M. J.; Qu, X. D.; Liu, W.*; Shen, B*. Biosynthesis of pharmaceutical natural
Selected Recent
products and their pathway engineering. In Breakthoughs and Perspectives in Organic Publications
Chemistry——Views Based on the Achievements in the First Decade of the 21th Century. Ding, K. L; Dai, L. X. Eds. John Wiley-VCH Verlag GmbH & Co. KGaA, 2011,
2. Qu, X. D.; Lei, C.; Liu, W.*, Transcriptome mining of active biosynthetic pathways and
their associated products in Streptomyces flaveolus. Angewandte Chemie
International Edition 2011, doi: 10.1002/anie.201103085.
3. Wu, J. Q.; Zhang, Q. L.; Deng, W.; Qian, J. C.; Zhang, S. L.; Liu, W.*, An artificial attB
site for specific recombination facilitates genetic manipulations towards improving the
erythromycin A production in an industrial Saccharopolyspora erythraea strain. Applied
and Environmental Microbiology 2011, doi: 10.1128/AEM.06034-11.
4. Zhang, Q.*; Liu, W.*, Complex biotransformations catalyzed by radical S-
adenosylmethionine enzymes. Journal of Biological Chemistry 2011, doi:
10.1074/jbc.R111.272690.
5. Zhang, Q.; Li, Y. X.; Chen, D. D.; Yu, Y.; Duan, L. A.; Shen, B.; Liu, W.*, Radical-
mediated enzymatic carbon chain fragmentation-recombination. Nature Chemical
Biology 2011, 7 (3), 154-160 (Featured in: BIOCHEMISTRY targeting tryptophan.
Science 2011, 311, 1366; Radical break-up, blissful make-up. Nat. Chem. Biol. 2011,
7, 133-134; Chemical biology: radical transformation. Nat. China 2011, doi:
10.1038/nchina.2011.9; Faculty of 1000 2011, post-publication peer review).
6. Liao, R. J.; Liu, W.*, Thiostrepton Maturation Involving a Deesterification-Amidation
Way To Process the C-Terminally Methylated Peptide Backbone. Journal of the
American Chemical Society 2011, 133 (9), 2852-2855.
7. Qu, X. D.; Jiang, N.; Xu, F.; Shao, L.; Tang, G. L.; Wilkinson, B.; Liu, W*., Cloning,
sequencing and characterization of the biosynthetic gene cluster of sanglifehrin A, a
potent cyclophilin inhibitor. Molecular Biosystems 2011, 7 (3), 852-861.
8. Zhang, Q.; Chen, D. D.; Lin, J.; Liao, R. J.; Tong, W.; Xu, Z. N.; Liu, W.*,
Characterization of NocL Involved in Thiopeptide Nocathiacin I Biosynthesis A 4Fe-4S
CLUSTER AND THE CATALYSIS OF A RADICAL S-ADENOSYLMETHIONINE
ENZYME. Journal of Biological Chemistry 2011, 286 (24), 21287-21294.
9. Zhang, Q.; Wu, J.; Qian, J.*; Chu, J.; Zhuang, Y.; Zhang, S.; Liu, W.*, Knocking out of
tailoring genes eryK and eryG in an industrial erythromycin-producing strain of
Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at
different conversion ratios. Letters in Applied Microbiology 2011, 52 (2), 129-137.
10. Ding, W.; Deng, W.; Tang, M. C.; Zhang, Q.; Tang, G. L.; Bi, Y. R.; Liu, W.*,
Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the
antitumor antibiotic azinomycin B. Molecular Biosystems 2010, 6 (6), 1071-1081.
11. Ding, W.; Lei, C.; He, Q. L.; Zhang, Q. L.; Bi, Y. R.; Liu, W.*, Insights into Bacterial 6-
Methylsalicylic Acid Synthase and Its Engineering to Orsellinic Acid Synthase for
Spirotetronate Generation. Chemistry & Biology 2010, 17 (5), 495-503.
12. Ding, Y.; Yu, Y.; Pan, H. X.; Guo, H.; Li, Y. M.; Liu, W.*, Moving posttranslational
modifications forward to biosynthesize the glycosylated thiopeptide nocathiacin I in
Nocardia sp ATCC202099. Molecular Biosystems 2010, 6 (7), 1180-1185.
13. Wang, J.; Yu, Y.; Tang, K. X.; Liu, W.; He, X. Y.*; Huang, X.; Deng, Z. X., Identification
and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic
Cyclothiazomycin in Streptomyces hygroscopicus 10-22. Applied and Environmental
Microbiology 2010, 76 (7), 2335-2344.
14. Yu, Y.; Guo, H.; Zhang, Q.; Duan, L. A.; Ding, Y.; Liao, R. J.; Lei, C.; Shen, B.; Liu,
W.*, NosA Catalyzing Carboxyl-Terminal Amide Formation in Nosiheptide Maturation
via an Enamine Dealkylation on the Serine-Extended Precursor Peptide. Journal of the
American Chemical Society 2010, 132 (46), 16324-16326.
15. He, Q. L.; Jia, X. Y.; Tang, M. C.; Tian, Z. H.; Tang, G. L.; Liu, W.*, Dissection of Two
Acyl-Transfer Reactions Centered on Acyl-S-Carrier Protein Intermediates for Incorporating Chembiochem 2009, 10 (5), 813-819.
16. Liao, R. J.; Duan, L.; Lei, C.; Pan, H. X.; Ding, Y.; Zhang, Q.; Chen, D. J.; Shen, B.; Yu, Y.*; Liu, W.*, Thiopeptide Biosynthesis Featuring Ribosomally Synthesized
Precursor Peptides and Conserved Posttranslational Modifications. Chemistry &
Biology 2009, 16 (2), 141-147 (Featured in: This week in techniques: Biosynthetic
pathway for the production of thiopeptide antibiotics. SciBX 2(10), 2009, Doi:
10.1038/scibx.2009.423; Thiopeptide antibiotic biosynthesis. Angew. Chem. Int. Ed.
2009, 48，6770-6773; Recent advances in thiopeptide antibiotic biosynthesis. Nat.
Prod. Rep. 2010, 27, 153-164).
17. Yu, Y.; Duan, L.; Zhang, Q.; Liao, R. J.; Ding, Y.; Pan, H. X.; Wendt-Pienkowski, E.; Tang, G. L.; Shen, B.; Liu, W.*, Nosiheptide Biosynthesis Featuring a Unique Indole
Side Ring Formation on the Characteristic Thiopeptide Framework. Acs Chemical
Biology 2009, 4 (10), 855-864 (Featured in:Recent advances in thiopeptide antibiotic
biosynthesis. Nat. Prod. Rep. 2010, 27, 153-164).
18. Liu, W.*; Yu, Y, Combinatorial Biosynthesis of pharmaceutical natural products.
manufacturing. In Biocatalysis for the Pharmaceutical Industry. Tao, J. H.; Lin, G. Q.;
Liese, A. Eds. John Wiley & Sons Pte Ltd, 2009, pp229-245.
19. Chen, Y.; Deng, W.; Wu, J. Q.; Qian, J. C.; Chu, J.; Zhuang, Y. P.; Zhang, S. L.*; Liu,
W.*, Genetic modulation of the overexpression of tailoring genes eryK and eryG
leading to the improvement of erythromycin a purity and production in
Saccharopolyspora erythraea fermentation. Applied and Environmental Microbiology
2008, 74 (6), 1820-1828.
20. Fang, J.; Zhang, Y. P.; Huang, L. J.; Jia, X. Y.; Zhang, Q.; Zhang, X.; Tang, G. L.; Liu,
W.*, Cloning and characterization of the tetrocarcin a gene cluster from
Micromonospora chalcea NRRL 11289 reveals a highly conserved strategy for
tetronate biosynthesis in spirotetronate antibiotics. Journal of Bacteriology 2008, 190
(17), 6014-6025.
21. Galm, U.; Wang, L. Y.; Wendt-Pienkowski, E.; Yang, R. Y.; Liu, W.; Tao, M. F.;
Coughlin, J. M.; Shen, B.*, In vivo manipulation of the bleomycin biosynthetic gene
cluster in Streptomyces verticillus ATCC15003 revealing new insights into its
biosynthetic pathway. Journal of Biological Chemistry 2008, 283 (42), 28236-28245.
22. Li, L.; Deng, W.; Song, J.; Ding, W.; Zhao, Q. F.; Peng, C.; Song, W. W.; Tang, G. L.*; Liu, W.*, Characterization of the saframycin a gene cluster from Streptomyces
lavendulae NRRL 11002 revealing a nonribosomal peptide synthetase system for
assembling the unusual tetrapeptidyl skeleton in an iterative manner. Journal of
Bacteriology 2008, 190 (1), 251-263.
23. Zhang, J.; Van Lanen, S. G.; Ju, J. H.; Liu, W.; Dorrestein, P. C.; Li, W. L.; Kelleher,
N. L.; Shen, B.*, A phosphopantetheinylating polyketide synthase producing a linear
polyene to initiate enediyne antitumor antibiotic biosynthesis. Proceedings of the
National Academy of Sciences of the United States of America 2008, 105 (5), 1460-
1465 (Featured in: Enediynes taught how to work better. C&E News 2007, 85, 25).
24. Zhao, Q. F.; He, Q. L.; Ding, W.; Tang, M. C.; Kang, Q. J.; Yu, Y.; Deng, W.; Zhang, Q.; Fang, J.; Tang, G. L.; Liu, W.*, Characterization of the azinomycin B biosynthetic
gene cluster revealing a different iterative type I polyketide synthase for naphthoate
biosynthesis. Chemistry & Biology 2008, 15 (7), 693-705 (Featured in: Antibiotic
biosynthesis:
Nat.
China
25. Liu, W.*; Zhang, S. L.; Chen, Y.; Deng, W.; Wu, J. Q., Improvement of Erythromycin a
purity and production at the fermentation stage of Saccharopolyspora erytharea by
metabolic engineering. Journal of Biotechnology 2008, 136 (Supplement 1): S21-S21.
26. Kennedy, D. R.; Gawron, L. S.; Ju, J. H.; Liu, W.; Shen, B.; Beerman, T. A.*, Single
chemical modifications of the C-1027 enediyne core, a radiomimetic antitumor drug,
affect both drug potency and the role of ataxia-telangiectasia mutated in cellular
responses to DNA double-strand breaks. Cancer Research 2007, 67 (2), 773-781.
27. Shen, B.*; Cheng, Y. Q.; Christenson, S. D.; Jiangi, H.; Ju, J. H.; Kwon, H. J.; Lim, S. K.; Liu, W.; Nonaka, K.; Seo, J. W.; Smith, W. C.; Standage, S.; Tang, G. L.; Van
Lanen, S.; Zhang, J., Polyketide Biosynthesis beyond the Type I, II, and III Polyketide
Synthase Paradigms: A Progress Report. In Polyketides: Biosynthesis, Biological
Activity, and Genetic Engineering, Rimando, A. M.; Baerson, S. R., Eds. 2007; Vol.
955, pp 154-166.
28. Van Lanen, S. G.; Oh, T. J.; Liu, W.; Wendt-Pienkowski, E.; Shen, B.*,
Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura
madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis.
Journal of the American Chemical Society 2007, 129 (43), 13082-13094.
29. Jia, X. Y.; Tian, Z. H.; Shao, L.; Qu, X. D.; Zhao, Q. F.; Tang, J.; Tang, G. L.*; Liu,
W.*, Genetic characterization of the chlorothricin gene cluster as a model for
spirotetronate antibiotic biosynthesis. Chemistry & Biology 2006, 13 (6), 575-585.
30. Shao, L.; Qu, X. D.; Jia, X. Y.; Zhao, Q. F.; Tian, Z. H.; Wang, M.; Tang, G. L.*; Liu,
W.*, Cloning and characterization of a bacterial iterative type I polyketide synthase
gene encoding the 6-methylsalicyclic acid synthase. Biochemical and Biophysical
Research Communications 2006, 345 (1), 133-139.
31. Liu, W.; Nonaka, K.; Nie, L. P.; Zhang, J.; Christenson, S. D.; Bae, J.; Van Lanen, S.
G.; Zazopoulos, E.; Farnet, C. M.; Yang, C. F.; Shen, B.*, The neocarzinostatin
biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving
two iterative type I polyketide synthases. Chemistry & Biology 2005, 12 (3), 293-302.
32. Van Lanen, S. G.; Dorrestein, P. C.; Christenson, S. D.; Liu, W.; Ju, J. H.; Kelleher, N.
L.; Shen, B.*, Biosynthesis of the beta-amino acid moiety of the enediyne antitumor
antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates. Journal of
the American Chemical Society 2005, 127 (33), 11594-11595.
33. Murrell, J. M.; Liu, W.; Shen, B.*, Biochemical characterization of the SgcA1 alpha-D-
glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor
antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces
globisporus to improve C-1027 production. Journal of Natural Products 2004, 67 (2),
206-213.
34. Christenson, S. D.; Liu, W.; Toney, M. D.; Shen, B.*, A novel 4-methylideneimidazole-
5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027
biosynthesis. Journal of the American Chemical Society 2003, 125 (20), 6062-6063.
35. Liu, W.; Ahlert, J.; Gao, Q. J.; Wendt-Pienkowski, E.; Shen, B.*; Thorson, J. S.*, Rapid
PCR amplification of minimal enediyne polyketide synthase cassettes leads to a
predictive familial classification model. Proceedings of the National Academy of
Sciences of the United States of America 2003, 100 (21), 11959-11963.
36. Shen, B.*; Liu, W.; Nonaka, K., Enediyne natural products: Biosynthesis and prospect
towards engineering novel antitumor agents. Current Medicinal Chemistry 2003, 10
(21), 2317-2325.
37. Zazopoulos, E.; Huang, K. X.; Staffa, A.; Liu, W.; Bachmann, B. O.; Nonaka, K.;
Ahlert, J.; Thorson, J. S.; Shen, B.; Farnet, C. M.*, A genomics-guided approach for
discovering and expressing cryptic metabolic pathways. Nature Biotechnology 2003,
21 (2), 187-190.
38. Liu, W.; Christenson, S. D.; Standage, S.; Shen, B.*, Biosynthesis of the enediyne
antitumor antibiotic C-1027. Science 2002, 297 (5584), 1170-1173 (featured in: New
enzymes in warhead. Nat. Biotechnol. 2002, 20, 984-985; Towards bioengineering
anticancer drugs. Chem. & Biol. 2002, 9, 956-958; Newly mapped bacterial genes
may contain blueprints for new anticancer drugs. The lancet, 2002, 360, 550; Path to
enediynes: Engineering of biosynthetic route could lead to better anticancer drugs.
C&E News, 2002, 80, 33; Chemical Highlights 2002: Biochemistry. C&E News, 2002,
80, 40).
39. Liu, W.; Shen, B.*, Genes for production of the enediyne antitumor antibiotic C-1027 in
Streptomyces globisporus are clustered with the cagA gene that encodes the C-1027
apoprotein. Antimicrobial Agents and Chemotherapy 2000, 44 (2), 382-392 (Featured
in: Enediyne research continues apace. C&E News, 2000, 78, 47-49).
40. Thorson, J. S.*; Shen, B.*; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J., Enediyne
biosynthesis and self-resistance: A progress report. Bioorganic Chemistry 1999, 27
(2), 172-188.
41. Wang, Z. L.; Zhao, Q. F.; Gao, S. H.; Chen, C. H.*; Liu, W.*, Construction of the New
Genetic Manipulation Method of Streptomyces lincolnensis and lmbQ Gene Function
Verification2010, 11, 186-190.
42. Zhang, H.; Zhao, Q. F.; Yu, Y.; Lu, D. N.*; Liu, W.*, A PCR approach for specific
accession of the genes encoding the reduction domain of non-ribosomal peptide
synthetase.2009, 34(3), 189-192.
43. Wang, Y.; Yu, Y.; Zhao, Q. F.; Kong, Y.*; Liu, W.*, Advance in Biosynthesis and
Combinatorial Biosynthesis of Natural Products.2008,
29(6), 275-282.
44. Liu, W.*, Progress of Biosynthesis of Enediyne Antitumor Antibiotics
2005, 27(3), 24-31.
45. Liu, W.*; Tang, G. L., Biosynthesis-Based Metabolic Engineering and Combinatorial
Biosynthesis2005, 25(1), 1-5.
46. Liu, W.; Li, Y.*, Study of biosynthesis gene cloning of novel antitumor antibiotic

Source: http://www.sioc-ccbg.ac.cn/tripos/upload/resume/[email protected]