List of major projects

Louis X. Tiefenauer
born 1954, married, 4 adult children, Swiss Biomolecular Research, Nanobiotechnology CH-5232 Villigen-PSI
Tel. +41-56-310-25-14; Fax.+41(56)310 52 88 Education and positions
- Diploma in biochemistry from Univ. Fribourg - Diploma for high school teaching from Univ. Fribourg 1978 - Postdoc at Children’s, Hospital Zürich - Product development for Baxter-Travenol - Group leader, research projects leader at PSI Research interests
General: Bio-material interfaces, functional aspects Previous interests: Influence of chemical structures on bio-recognition (conjugation chemistry), single molecule measurements (AFM, biophysics), electron transfer in molecular assemblies (biosensing, electrochemistry), tissue targeting (MRI, radiotherapy, biomedical research), surface patterning (neurochips, biomaterials), nanotechnology (nanostructures fabrication, material science techniques) for bioanalytical applications (nano-biotechnology) Present interest: Free-standing lipid bilayers in nanopores to establish functional assays for membrane proteins for drug discovery applications Ethics and safety
Research Integrity: Consultant, guidelines for good scientific practice, implementation at PSI, coordination of research integrity issues in ETH domain Communication: (Nano)technology benefit-risk debate, science public events Safety: nano-safety, institutional safety List of major projects
initiated (i), project leader Louis Tiefenauer 1. Clinical research 1981-1982
Research project at Children Hospital Zürich: Distribution of radiolabeled antibiotics in cell
compartments. Biochemical research on chronic granulomatous disease.
2. Radioimmunoassay kit developments for various steroids 1983-1986
Product development for industry (Baxter-Travenol): Organic synthesis, radiolabeling and
coating techniques, analytical biochemistry; quality control & customer service.
3. Investigations on enzyme immunoassays (ELISA) 1986-1988 (i)
Research project at PSI: ELISA for estradiol based on the avidin-biotin system, conjugate
synthesis and evaluation, non specific binding reduction.
4. Radiotherapy using Rhenium in liposomes 1986-1989
Research project at PSI: Radioisotope production, liposome production, radiopharmacy.
5. Amperometric immunosensors using glassy carbon electrodes 1988-1991 (i)
Research project at PSI: Immobilization techniques, electrochemistry.
6. Contrast agent for magnetic resonance imaging (MRI) 1991-1998
Research project SNF at PSI:
Inorganic chemistry, Immobilization of antibodies, analytical
biochemistry, animal experiments, MR-imaging and spectroscopy
7. Amperometric immunosensors based on nanostructured silicon chips 1993-2008 (i)
Research projects SPP Biotech, in collaborations:
(1) Immobilization of proteins on silicon, preparation and analysis of nanostructured surface,
electrochemical detection, integration of chips into micro-machined flow injection analysis (FIA) systems. (2) Feasibility of an automated immunosensor device for penicillin in milk. (3) Applications for bio-molecular electronics and combination with fluorescence sensors. 8. Biochemical recognition of individual molecules (BioAFM) 1996-2000 (i)
Research project NFP36, in coll.: Immobilization of individual, engineered antibody molecules
on tips, atomic force microscopy (AFM) and spectroscopy technologies.
9. Neurite growth on biofunctionalized microstructured silica 1997-1999 (i)
Research project SPP Biotech, in coll.: Fabrication and bio-functionalization of microstructured
silica, neuron culturing and electrophysiological detection.
10. Nanostructured chips for the analysis of individual membrane proteins 2000-2002 (i)
Feasibility study KTI/TopNano21, in coll.: Fabrication processes for nanostructured chips
11. Production of nano-patterned materials for cell adhesion studies 2001-2002 (i)
Technology-oriented project KTI/TopNano21, in coll.: production of contrast nanopatterns
12. Functional assays for membrane proteins on nanopore array supports 2004-2007 (i)
Technology-oriented KTI-project, in coll
., based on a patent: feasibility and research study
13. Measuring protein-mediated ion transport across planar lipid bilayers 2005-2008 (i)
Research project PSI:
studies on membrane protein functions using nanopore array chips
14. Functional assays for membrane proteins using nanostructured supports 2008-2011 (i)
Research project ASMENA: EU 7PF, in coll.: membrane protein experession reconstitution, lipid
bilayer formation, microfluidics (Patent), patch-clamp technology
List of referred publications
Louis Tiefenauer (status 24.01.2012) (cited: total 1443, in average 26.2 per paper; h-index: 22) 1. Chronic granulomatous disease: Effect of sulfamethoxazole/trimethoprim on neutrophil microbicidal function
R.A. Seger, S. Baumgartner, L.X. Tiefenauer & F.K. Gmünder
Helv. Paediat. Acta 36, 579-588 (1981).
2. 3 families with partial cytochrome B definciency - a new cause of recurrent bacterial infactions
R. Seger, L. Tiefenauer, M. Bohler, T. Matsunaga, B. Belohradsky, U. Schaad, V. Wahn,
W.H. Hitzig
European J. Pediatrics 139, 311 (1982).
3. Chronic granulomatous disease due to granulocytes with abnormal NADPH oxidase activity and deficient cytochrome-b
R.A. Seger, L.X. Tiefenauer, T. Matsunaga, A. Wildfeuer & P.E. Newburger
Blood 61, 423-428 (1983).
4. Glycogenosis Ib: Neutrophil microbicidal defects due to impaired hexose monophosphat shunt
R.A. Seger, B. Steinmann, L.X. Tiefenauer T. Matsunaga & R. Gitzelmann
Pediatric Research 18, 297-299 (1984).
5. Prevention of bridge binding effects in haptenic immunoassay systems exemplified by an iodinated radioimmunoassay for melatonin
L.X. Tiefenauer & R.Y. Andres
J. Immunol. Methods 74, 293-298 (1984).
6. Congenital acatalasemia: a study of neutrophil functions after provocation with hydrogen peroxide
T. Matsunaga, R. Seger, P. Höger, L.X. Tiefenauer & W. Hitzig
Pediatric Research 19, 1187-1190 (1985).
7. Dehydroepiandosterone-sulfat secretion and state of adrenal function in the rat I. Investigation of basic endocrine aspects in the well-fed state after dexamethasone,
ACTH, metyrapone and hCG treatment
T. Remer, K. Pietrzik, H, Wieland & L.X. Tiefenauer
J. Clin. Biochem. Nutr. 2, 141-152 (1987).
9. Antibody coating using various avidin-biotin complexes employed to an enzyme immunoassay for estradiol
L.X. Tiefenauer & D. Bodmer
Fres. Z. Anal. Chem. 330, 342 (1988).
10. Immunoscintigraphic localization of inflammatory lesions: Concept, radiolabelling and in vitro testing of a granulocyte specific antibody
R.Y. Andres, P.A. Schubiger, L. Tiefenauer, K. Seybold, J.T. Locher, J.-P. Mach & F.
Eur. J. Nucl. Med. 13, 582-586 (1988).
11. Prevention of bridge binding in immunoassay: A general estradiol tracer structure L.X. Tiefenauer, D.M. Bodmer, W. Frei & R.Y. Andres
J. steroid Biochem. 32, 251-257 (1989).
12. Antigen- versus antibody immobilized ELISA procedures using a biotin-estradiol conjugate
D.M. Bodmer, L.X. Tiefenauer & R.Y. Andres
J. steroid Biochem. 33, 1161-1166 (1989).
13. Biotinyl-estradiol derivatives in enzyme immunoassays: Structural requirement for optimal antibody binding
L.X. Tiefenauer & R.Y. Andres
J. steroid Biochem. 35, 633-639 (1990).
14. Treatment of antibodies to reduce non-specific binding in immunoassays using the avidin-biotin complex
D.M. Bodmer & L.X. Tiefenauer
J. Immunoassay 11, 139-145 (1990).
15. A lipophilic complex with 186Re/188Re incorporated in liposomes suitable for radiotherapy
U. Häfeli, L.X. Tiefenauer, P.A. Schubiger & H.G. Weder
Nucl. Med. Biol. 18, 449 - 454 (1991).
16. Covalent immobilization of avidin on glassy carbon electrodes as the basis for multivalent biosensors
U.R. Achtnich, L.X. Tiefenauer & R.Y. Andres
Biosensors & Bioelectronics 7, 279 - 290 (1992).
17. Antibody-magnetite nanoparticles: In vitro characterization of a potential tumor specific contrast agent for magnetic resonance imaging
L.X. Tiefenauer, G. Kühne & R.Y. Andres
Bioconjug. Chem. 4, 347 - 352 (1993).
18. Use of biotinyl-estradiol derivative to demonstrate estradiol-membrane binding sites on adherent human breast cancer MCF-7 cells
P. Germain, P. Metezeau, L.X. Tiefenauer, H. Kiefer, M. H. Ratinaud & G. Habrioux
Anticancer Research 13, 2347 - 2354 (1993).
19. Immobilization of streptavidin for immunosensors on nanostructured surfaces S. Kossek, C. Padeste & L. Tiefenauer
J. Mol Recogn. 9, 485-487 (1996).
20. In vivo evaluation of magnetite nanoparticles for use as a tumor contrast agent in MRI L.X. Tiefenauer, A. Tschirky, G. Kühne & R.Y. Andres
Magnetic Resonance Imaging 14, 391-402 (1996).
21. Fabrication and characterization of nanostructured gold electrodes for electrochemical biosensor devices
C. Padeste, S. Kossek, H.W. Lehmann, C. Musil, J. Gobrecht & L. Tiefenauer
J. Electrochem. Soc. 143, 3890-3895 (1996).
22. Towards amperometric immunosensor devices L. Tiefenauer, S. Kossek, C. Padeste & P. Thiébaud
Biosensors & Bioelectronics 12, 213-223 (1997).
23. Localization of individual biomolecules on sensor surfaces S. Kossek, C. Padeste, H. Siegenthaler & L. Tiefenauer
Biosensors & Bioelectronics 13, 31-43 (1998).
24. Antigen binding forces of individually addressed single-chain Fv antibody molecules R. Ros, F. Schwesinger, D. Anselmetti, M. Kubon, R. Schäfer, A. Plückthun & L.
Proc. Natl. Acad. Sci. USA 95, 7402-7405 (1998).
25. Amperometric immunosensing using microperoxidase MP-11 antibody conjugates C. Padeste, A. Grubelnik, L. Tiefenauer
Anal. Chim. Acta 374, 167 -176 (1998).
26. Micro- and nanotechnology in biosensor research L. Tiefenauer, C. Padeste
Chimia 53, 62-66 (1999).
27. Neurite outgrowth on microstructured surfaces functionalized by a neural adhesion protein
H. Sorribas, L. Leder, D. Fitzli, C. Padeste, T. Mezzacasa, P. Sonderegger & L.
J. Mater. Sci. Mater. Med. 10, 787-791 (1999).
28. Hochempfindliche Immunoassays für den Nachweis von Penicillinen A. Grubelnik, C. Padeste, L. Tiefenauer
Swiss Patent Application No. 1764/99 (1999).
29. Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rate
F. Schwesinger, R. Ros, T. Strunz, D. Anselmetti, H.-J. Güntherodt, A. Honegger, L.
Jermutus, L. Tiefenauer and A. Plückthun
Proc. Natl. Acad. Sci. USA 97, 9972-9977 (2000).
30. Ferrocene-avidin conjugates for bioelectrochemical applications C. Padeste, A. Grubelnik & L. Tiefenauer
Biosensors & Bioelectronics, 15, 431-438 (2000).
31. Highly sensitive enzyme immunoassay (EIA) for detection of ?-lactam antibiotics A. Grubelnik, C. Padeste, L. Tiefenauer
Food & Agricult. Immunol. 13, 161-169 (2001).
32. Adhesion proteins for a tight neuron-electrode contact H. Sorribas, D. Braun, L. Leder, P. Sonderegger & L. Tiefenauer
J. Neuroscience Methods 104, 133-141 (2001).
33. Biointerface analysis on a molecular level L. Tiefenauer & R. Ros
Colloids & Surfaces B: Biointerfaces 23, 95-114 (2002).
34. Photolithographic generation of protein micropatterns for neuron culture applications H. Sorribas, C. Padeste & L. Tiefenauer
Biomaterials 23, 893-900 (2002).
35 Redox labeled avidin for enzyme sensor architectures C. Padeste, B. Steiger, A. Grubelnik & L. Tiefenauer
Biosensors & Bioelectronics 19, 239-247 (2003).
36. High volume fabrication of customizued nanopore membrane chips L.J. Hederman, B. Ketterer, D. Bächle, F. Glaus, B. Haas, H. Schift, K. Vogelsang, J.
Gobrecht, L. Tiefenauer, O. Dubochet, P. Surbled & T. Hessler
Microelect. Engineering 67-68, 208-213 (2003).
37. Charge transport effects in ferrocene-streptavidin multilayers immobilized on electrode surfaces
B. Steiger, C. Padeste, A. Grubelnik & L. Tiefenauer
Electrochimica Acta 48, 761-769 (2003).
38. L. Tiefenauer „Functional assays for membrane proteins in lipid bilayers on nanopore
array supports“ Patent appl. EP01 Nr. 04 002 119.8, 31. 01. 2004. 39. Molecular assembly of redox-conductive ferrocene-streptavidin – towards bioelectrochemical devices
C. Padeste, B. Steiger, A. Grubelnik & L. Tiefenauer
Biosensors & Bioelectronics 20, 545-552 (2004).
L. Tiefenauer
Technologiefolgenabschätzung – Theorie und Praxis, 13. Jg, Nr.2, 52-57 (2004).
41. Simultneously amplified electrochemical and surface plasmon resonce optical detection of DNA hybridizsation based on ferrocene-streptavidin conjugates
J. Liu, S. Tian, L. Tiefenauer, P.E. Nielson & W. Knoll
Anal. Chem. 77, 2756- 2761 (2005).
42. Study on PNA-DNA hybridization based on labeled-streptavidin by voltammetry and surface plasmon fluorescence spectroscopy
J. Liu, L. Tiefenauer, S. Tian, P.E. Nielsen & W. Knoll
Anal. Chem. 78, 470-476 (2006).
43. A mixture of ferritin and magnetite nanoparticles mimics magnetic properties of human brain tissue
F. Brem, L. Tiefenauer, A. Fink, J. Dobson, A.M. Hirt
Phys. Rev. B 73, 224427, 1-6 (2006)
44. Nanopore arrays for stable and functional free-standing lipid bilayers X. Han, M. DiBerardino, F. K. Winkler, L.X. Tiefenauer
Advanced Materials 19, 4466-4470 (2007).
45. Ethics of nanotechnology in medicine: challenges and promises L. Tiefenauer
Nanobiotechnology, online 2, 1551-1294 (2007).
46. O. Azzaroni, M. Mir, L. Tiefenauer, W. Knoll
Electrochemical rectification with redox-labeled supramolecular bioconjugates: molecular building blocks for the construction of biodiodes Langmuir, 24, 2878-2883 (2008). 47. L. Tiefenauer, A. Studer
Nano for Bio: Nanopore arrays for stable and functional lipid bilayer membranes, minireview , Biointerphases 3, FA74-FA79 (2008). 48. M. Mir, M. Álvarez, O. Azzaroni, L. Tiefenauer, W. Knoll
Molecular architectures for electrocatalytic amplification of oligonucleotide hybridization
Anal. Chem. 80, 6554-6559 (2008).
49. A. Studer, X. Han, F.K. Winkler, L.X. Tiefenauer
Monitoring insertion of melittin and hemolysin in lipid bilayers suspended in nanopores Colloids and Surfaces: Biointerfaces B 73, 325-331 (2009). 50. A. Studer, S. Demarche, D. Langenegger, L. Tiefenauer
Integration and recording of a reconstituted voltage-gated sodium channel in planar lipid bilayers Biosensors & Bioelectronics 26, 1924-1928 (2011). 51. Recording of ion channels: a critical review S. Demarche, K. Sugihara, T. Zambelli, L. Tiefenauer, J. Vörös
Analyst, 136, 1077-1089 (2011).
52. A method for producing a polymer-based microfluidics system for bioanalytics using biological membranes, E. Müller, I. Imhof, L. Tiefenauer, H. Schift,
European Patent application EP11163 710.4, 26. 04. 2011.



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